This enzyme allows amplification of simple and complex dna templates over a large range of target sizes and provides 6x higher fidelity over taq. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. Taq dna polymerase is a thermostable dna polymerase that possesses a 5. A protocol for the use of gotaq dna polymerase with 5x green gotaq reaction buffer and 5x colorless gotaq reaction buffer. Gotaq products offer a choice of taq polymerase formulations for basic pcr, hotstart pcr and longrange pcr. Gotaq flexi dna polymerase is a proprietary formulation of taq dna polymerase that gives robust amplification equal to and in some cases superior to that of standard taq. The enzyme is a taq dna polymerase with buffers designed for enhanced amplification. The enzyme has terminal transferase activity which results in the addition of a single nucleotide adenosine at 3 end of the extension product. The 5x green gotaq reaction buffer, contains two dyes. Gotaq hot start master mixes are allinone master mixes just add template and primers that contain the highperformance gotaq dna polymerase bound to a proprietary antibody that blocks polymerase activity until the reaction is heated at 9495c for two minutes. Pfu dna polymerase is a highfidelity, thermostable enzyme of approximately 90kda isolated from pyrococcus furiosus. Gotaq taq dna 5x green colorless flexi reaction buffers.
Dna amplified using gotaq dna polymerase with green gotaq reaction buffer can be loaded directly onto agarose gels, eliminating the need to add loading dye or. Gotaq dna polymerase contains gotaq dna polymerase, 5x green gotaq reaction buffer and 5x colorless gotaq reaction buffer. Promega corporation 2800 woods hollow road madison, wi 537115399 usa telephone 6082744330 toll free 8003569526 fax 6082772516 internet. Gotaq dna polymerase m300 protocolpdf 207 kb english. Dreamtaq hot start green dna polymerase 200 u 500 u 2,500 u 4 x 2,500 u ep1711 ep1712. Gotaq dna polymerase is tested for performance in the polymerase chain reaction pcr using 1. Gotaq green master mix the wolfson centre for applied structural. The recombinant protein will be purified from an e. Promega pcr systems, enzymes and reagents are proven in pcr to ensure reliable. Pcr amplification an introduction to pcr methods promega.
Taq polymerase for robust pcr with and directtogel convenience. The proven robust amplification using gotaq polymerase is now available for longrange pcr up to 30kb gdna. Gotaq hot start polymerasea,b contains highperformance. Gotaq green master mix product information promega corporation. Gotaq dna polymerase is a proprietary formulation of taq polymerase that gives robust amplification equal to and, in some cases, superior to that of standard taq the 5x gotaq green and colorless reaction buffers supplied with gotaq dna polymerase contain mgcl 2 at a concentration of 7. Pcr protocol for taq dna polymerase with standard taq buffer m0273 protocols. Gotaq dna polymerase, promegas new formulation of taq. Gotaq green master mixa,b is a premixed readytouse solution containing bacterially derived taq dna polymerase, dntps, mgcl2 and. The enzyme replicates dna at 75c, catalyzing the polymerization of nucleotides in the 5.
It comes in a variety of formats designed to provide maximum flexibility, control and convenience. Gotaq dna polymerase is supplied in 2x green gotaq reaction buffer ph 8. Taq dna polymerase pcr buffer this product is compatible for use in the polymerase chain reaction pcr process claimed in patents owned by roche molecular systems, inc. Pdf production and evaluation of taq dna polymerase. Gotaq g2 is a fulllength, recombinant taq polymerase supplied with buffers designed for enhanced amplification. Promega corporation 2800 woods hollow road madison, wi 537115399 usa telephone 6082744330 toll free 8003569526 fax 6082772516 internet pcr satisfaction guarantee promegas pcr systems, enzymes and reagents are proven in pcr to ensure reliable, high performance results.
The enzyme is a fulllength form of taq dna polymerase that exhibits 5. Buffer choice we recommend using the 5x green gotaq flexi buffer in any amplification reaction that. Gotaq dna polymerase product information promega corporation. Gotaq dna polymerase, promegas new formulation of taq dna polymerase, is supplied with two buffersgreen gotaq reaction buffer and colorless gotaq reaction buffer. Commercial producers have sinced cloned and expressed the enzyme in e. What is taq polymerase taq dna polymerase is a thermostable enzyme derived from the thermophilic bacterium thermus aquaticus. The gotaq g2 hot start master mixes are readytouse mixes containing all necessary components gotaq g2 hot start polymerase, buffer, dntps and optimized magnesiumyou only need to add primer and template, and go. The supplied 5x green and colorless flexi reaction buffers do not contain magnesium. Pcr the polymerase chain reaction pcr is a powerful and sensitive technique for dna amplification 1. The enzyme is a taq dna polymerase supplied in a proprietary formulation containing 50% glycerol with buffers designed for enhanced amplification. Green buffer allows direct gel analysis after amplification. Faststart taq dna polymerase is a versatile enzyme that can be used in a wide variety of applications and on multiple instrument platforms. The extension reaction is typically performed at the optimal temperature for taq.
Purification of taq dna polymerase for 1 liter culture modified from the protocol presented in f. Gotaq green master mix protocol promega corporation. Production and evaluation of taq dna polymerase article pdf available in journal of the medical association of thailand chotmaihet thangphaet 80 suppl 1. Gotaq dna polymerasea,b is a taq dna polymerase supplied in a proprietary formulation containing 50% glycerol with buffers designed for. Recombinant taq is a 94kda enzyme with an optimal 53 polymerase activity between 7580 c in the presence of a magnesium cofactor. Platinum ii taq hotstart dna polymerase thermo fisher. Both buffers contain mgcl 2 at a concentration of 7. It has a 53 dna polymerase activity and a 53 exonuclease activity. Promegas pcr systems, enzymes and reagents are proven in pcr to ensure reliable, high performance results. Our teams are in regular contact with suppliers and distributors worldwide to manage inventory of raw materials to ensure continued availability. The enzyme is a fulllength form of taq dna polymerase that exhibits 53 exonuclease activity. Dna amplified using gotaq dna polymerase with green gotaq reaction buffer can be loaded directly onto agarose gels, eliminating the need to add loading dye or buffer to the pcr sample before.
Gotaq hot start polymerase product information promega. The glycerolfree product formulation is further purified to remove glycerol, making the enzyme suitable for further manufacture processing and lyophilization. Taq polymerase is a thermostable dna polymerase i named after the thermophilic eubacterial microorganism thermus aquaticus, from which it was originally isolated by chien et al. Gotaq mdx dna polymerase is a fulllength form of taq dna polymerase that exihibits 5. The polymerase activity is blocked at ambient temperatures and restored after the initial denaturation step at 94c. The polymerase chain reaction pcr was developed by chemist kary mullis in the 1980s, as a means to make many copies of dna fragments. Enhanced performance over standard taq dna polymerase. The hotstart polymerase for everyday research new thermo scientific dreamtaq hot start dna polymerase offers a great balance between performance and value. It is commonly used to amplify dna fragments in pcr. Gotaq g2 flexi dna polymerase is supplied in a proprietary formulation containing 50% glycerol with buffers designed for enhanced amplification.
Gotaq dna polymerase a,b is a taq dna polymerase supplied in a proprietary formulation containing 50% glycerol with buffers designed for enhanced amplification. Gotaq g2 hot start taq is the second generation of gotaq products. Promega manufacturing and delivery systems continue to be fully operational during the covid19 outbreak. A universal primer annealing feature reduces optimization steps and allows for cocycling of all assays. Genscript taq dna polymerases are highly thermostable recombinant dna polymerases and ideally suited for routine pcr reactions. It is frequently used in the polymerase chain reaction pcr, a method for greatly amplifying the quantity of short segments of dna. Pcr reactions prepared with these buffers can be loaded directly onto. Polymerase activity is restored during the initial denaturation step, when amplification reactions are heated at 9495c for two minutes, allowing hotstart pcr in which polymerase activity is inhibited. Invitrogen platinum ii taq hotstart dna polymerase is designed to get you to your research destination, faster. Promega corporation 2800 woods hollow roadmadison, wi 537115399 u. It is supplied with 10x standard taq reaction buffer, which is detergentfree and designed to be compatible with existing assay systems. Pcr protocol for taq dna polymerase with standard taq buffer m0273. The highperformance gotaq g2 dna polymerase is bound to a proprietary antibody that blocks polymerase activity. The 5x green gotaq reaction buffer has two dyes a blue dye and a yellow dye that separate during electrophoresis to show migration progress.
The enzyme is in a recombinant form, expressed in e. Promegas pcr systems, enzymes and reagents are proven in pcr to ensure. Secondgeneration, highperformance gotaq g2 dna polymerase with mgfree buffers. Gotaq long pcr master mix contains the highperformance gotaq hot start polymerase in a specially formulated mixture with a proprietary thermal stable proofreading polymerase. Gotaq g2 dna polymerase product informationpdf 191 kb english. First isolated from the bacterial thermophile thermus aquaticus, the taq polymerase has become the staple enzyme in molecular biology. Invitrogen platinum ii taq hotstart dna polymerase is an engineered taq dna polymerase that shows increased resistance to reaction inhibitors originating from sample material or dna purification steps. The 5x green gotaq flexi reaction buffer has two dyes a blue dye and a yellow dye that separate during electrophoresis to show migration progress.
Provided with green and colorless reaction buffers. Shop online for a wide selection of promega gotaq dna polymerase taq dna polymerase. Gotaq g2 hot start taq is bound to a proprietary antibody that blocks polymerase activity until the reaction is heated to 9495c during initial denaturation. The 5x green gotaq flexi buffer contains two dyes a blue. Gotaq flexi dna polymerasea,b is supplied in a proprietary formulation containing 50% glycerol with buffers. Gotaq dna polymerase taq dna polymerase 5x green gotaq reaction buffer5x colorless gotaq reaction buffer5x green gotaq reaction buffer2. Recombinant taq dna polymerase purification in todays lab we will purify recombinant taq dna polymerase and assay for its function. Taq dna polymerase is a thermostable enzyme that synthesizes dna from singlestranded templates in the presence of dntps and a primer. Gotaq dna polymerasetaq dna polymerase5x green gotaq reaction buffer5x colorless gotaq reaction buffer.
Because taq dna polymerase is a nonproofreading polymerase, pcr products generated with taq dna polymerase will contain a singlenucleotide 3. Toll free in the usa 8003569526 telephone 6082744330 internet usage information b. Highperformance taq dna polymerase, nucleotides dntps, buffers and master mixes provide increased reliability and consistency for routine endpoint pcr. Gotaq g2 dna polymerase is supplied with 5x green gotaq reaction buffer and 5x colorless gotaq reaction buffer. Gotaq dna polymerase is a proprietary formulation of taq polymerase that gives robust amplification equal to and, in some cases, superior to that of standard taq. The enzyme consists of a single polypeptide with a molecular weight of 94 kda. Day 1 start 3ml overnight culture of taq from glycerol stock in lbamp 75ngul day 2 add 1ml of overnight culture to 1l of lbamp.
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